Selection in Vitro for NaCI-tolerance in Vitis rupestris Scheele
Identifieur interne : 000287 ( France/Analysis ); précédent : 000286; suivant : 000288Selection in Vitro for NaCI-tolerance in Vitis rupestris Scheele
Auteurs : L. Lebrun [France] ; K. Rajasekaran ; M. G. Mullins [Australie]Source :
- Annals of Botany [ 0305-7364 ] ; 1985-12.
Abstract
Suspension cultures were made from the vegetative tissues of anthers of the grape rootstock Vitis rupestris Scheele cv. Rupestris St George. Cell lines and somatic embryos were isolated which grew in agitated liquid media containing up to 150 mM NaCl. Exposure to NaCl (50–100 mM) promoted outgrowth of secondary somatic embryos but inhibited development of plantlets. Within somatic embryos the cotyledon and hypocotyl tissues were more tolerant of NaCl than root tissues. Elongation of the radicle in embryos in the presence of NaCl (50 mM) was associated with necrosis and death of root tissue but hypocotyls and cotyledons remained viable and continued to produce callus and secondary somatic embryos. The usefulness of the cell culture approach in selecting for salt tolerance is questioned.
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DOI: 10.1093/oxfordjournals.aob.a087064
Affiliations:
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